Spin labels

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High-field electron spin resonance of spin labels in membranes.

High-field electron spin resonance (ESR) spectroscopy is currently undergoing rapid development. This considerably increases the versatility of spin labelling which, at conventional field strengths, is already well established as a powerful physical technique in membrane biology. Among the unique advantages offered by high-field spectroscopy, particularly for spin-labelled lipids, are sensitivi...

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Motion of steroid spin labels in membranes.

Steroid spin labels have been found to undergo rapid and sometimes anisotropic rotational motion in membranes and phospholipid vesicle preparations. N-oxyl-4',4'-dimethyloxazolidine derivatives of 5alpha-androstan-3-one-17beta-ol, 5beta-androstan-3-one-17beta-ol, and 5alpha-androstan-3-one were prepared according to the procedure described by Keana. The paramagnetic five-membered ring is rigidl...

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Interaction between spin labels and DPPC vesicles.

1H NMR studies on DPPC vesicles labeled with the spin labels (1,14) or (12,3) have shown that both of the spin labels influence the fatty acid side chains as well as the choline head groups. The spin label (12,3) affects, naturally, the head groups stronger than the spin label (1,14), while the reversed effect can be observed at the side chains. This effect progresses with time after vesicle pr...

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Physics and chemistry of spin labels.

II. ANALYSIS OF THE MAGNETIC RESONANCE SPECTRA 94 OF BIOLOGICAL SYSTEMS CONTAINING SPIN LABELS A. Orientation of spin labels in simple host crystals 95 B. Orientation of spin labels in protein crystals 100 C. Orientation of spin labels in biological membranes 103 D. Anisotropic motion of spin labels n o E. Conformational changes in labeled macromolecules 114 F. Nuclear relaxation in biological ...

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Interaction of nitroxide spin labels with chloroplasts.

Chloroplasts isolated from oats eliminated the electron spin resonance (ESR) signals from spin labels in white light and partially restored them in far-red light. Only the white light-mediated reaction was blocked by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). In contrast, oat (Avena sativa L. cv. Garry and Park) leaf mesophyll protoplasts oxidized the spin labels in both white and far-red ...

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ژورنال

عنوان ژورنال: Nature

سال: 1977

ISSN: 0028-0836,1476-4687

DOI: 10.1038/266662b0